Haemon’s Speech Analysis

Haemon’s Speech Analysis Pride and stubbornness can be harmful things, and Haemon touches upon this within his speech to Creon as he attempts to dissuade his father from taking Antigone’s life. Using rhetorical devices such as tone, ethical, emotional, logical appeal, and metaphor, Haemon manages to make an impact on Creon’s eventual decision as he speaks in Antigone’s defense.Starting at the beginning of his speech, Haemon ventures to convince Creon to change his mind about his harsh ruling against Antigone, not by raising his voice or attempting to beat his â€Å"earnest† views into his father, but by using great tact and endeavoring to find a common ground with Creon, possibly trying to understanding his father’s position on the matter at hand. First, Haemon alleges that reason is â€Å"God’s crowning gift to man†, and that his father is â€Å"right† to â€Å"warn [him]† against losing that reason.He even keenly asserts that he never wants to say that â€Å"[Creon] has reasoned badly†, continuing on to make other mindful concessions. However, when this thoughtful mode of speaking doesn’t take any immediate effect, Haemon starts to become more candid with his words, implying (although not directly) that Creon’s actions are unjust and stubborn, taking care to adopt a point of view that is constructively criticizing as opposed to being completely insulting. He rationally cautions his father against the mindset of him having all of â€Å"the power†, noting that if Creon continues upon that path he will eventually â€Å"turn out† an â€Å"empty† man.He states that even people like his father must be able to stop, listen, and learn from others—not be completely fixed and â€Å"unchangeable†, because no man in the world is completely infallible. A few lines later in the speech, in addition to his amiable tone, Haemon also begins to use some emotional appeal, acknowledging his love and respect for his father, affirming that â€Å"nothing [is] closer to [him] than [his father’s] happiness†, and that he â€Å"values his father’s fortune† as much as his own.Haemon also logically recognizes his youthfulness and lack of wisdom as well, although he astutely uses what the people of Thebes have been â€Å"muttering and whispering† to support his argument, using aspects of ethical appeal to attest that Antigone’s innocence is what the people of his father’s city would want, realizing that while Creon would like first and foremost to be a good ruler, e also wishes to be well-liked by his people. Then coming to closing lines of his speech, Haemon begins to weave in clever metaphors here and there, first comparing a â€Å"stubborn tree† being â€Å"torn up† and then a â€Å"fast† and â€Å"never-slackened sail† going â€Å"head over heels and under† the water to his father, indicating that this is the type of thing what will happen if Creon thinks that he alone â€Å"can be right†.And in the very end, Haemon leaves his father with some loaded words, advising that Creon listen to him, because while â€Å"men should be right by instinct†, â€Å"[they] are all too likely to† be led astray, and that the smartest thing would be to learn from those who can are willing to teach them how to stay, or even make their own paths, in life.

Airport Security: the Post 9/11 Age

Airport Security: The Post 9/11 Age Airport security in the world we live in today is drastically different then the security we knew before the events of September 11, 2001, when four passenger airliners were high-jacked over the skies of the United States, causing a global terror pandemic that still has long lasting effects today. We will look at, discuss and break down some of the key features of airport security in Canada as well as the United States, that have been improved, as well as certain security programs and features which were freshly implemented as a direct result.We will discuss â€Å"no-fly lists,† personnel training and armament, and the Canadian Air Transport Security Authority or â€Å"CATSA,† including their security screening techniques and procedures (screening, responsibilities, Air Marshalls). The â€Å"No-Fly List† was created shortly after the events of 9/11, by the United States government’s Terrorist Screening Center (TSC). The list includes the names of people who are deemed unsafe, or a threat to Airline security and are no longer permitted to board a commercial airline for the purpose of travel coming in or out of the United States.The list now contains roughly 10,000 names (2011), the number people on the list fluctuates based on threat, and intelligence reporting. Canada also has a similar list called the â€Å"Passenger Protect,† a similar initiative to that of the United States list, implemented on June 18, 2007 in order to identify people who could be a threat to the security of aviation, and prevent them from boarding Canadian domestic flights as well as International flights to or from Canada.The Passenger Protect program has two main parts: a set of â€Å"Identity Screening Regulations† which requires all passengers 18 years of age and older to present a valid form of government-issued Identification in order to board a flight, as well as a â€Å"Specified Persons List† whic h has a name, birth date, and gender of the individuals believed to pose a security threat. The list contains roughly 1,250 names. Individuals who have been denied boarding and are in fact on the list can submit an appeal to a branch of Transport Canada, called the Office of Reconsideration.The program works by the government supplying the Specified Persons List to Airlines, who then compare names of people on the list with individuals who intend on boarding flights. When the airline finds a name match, they then reference the individual’s government-issued identification to confirm. The identification is then once more confirmed in person at the airport check-in area, when a confirmation has been established Transport Canada is immediately notified.Training is also provided by Transport Canada to the airlines to teach staff and agents how to implement the ID verification process, and establish procedures to ensure the rights of the passengers are respected. Of course with a system like this, controversy is brought up by civil liberty organizations citing their concerns for civil liberties, racial profiling, privacy, and the perceived failure of the no-fly list created in the United States. In the United States, several anomalies’ referred to as â€Å"false positives† have risen.A â€Å"false positive† happens when an individual who is actually not on the no-fly list, has a name matching or similar to a name on the list. One notable case of a false positive includes a United States Marine in April of 2006, who was flying home from Iraq when he was prohibited from boarding his flight home, as his name matched one on the no-fly list. The lists, although very controversial both here in Canada and in the United States play an important role in establishing airport/airline safety and security, and ensuring an event such as the attacks on September 11, 2001 will not re-occur.Secondly, the way Security Personnel are trained and armed has b een drastically affected in the post 9/11 world. In the United States, immediately after the attacks for several weeks to months afterwards, fully armed guards, either Local Police or National Guard would patrol airports in full tactical gear wielding assault rifles or tactical shotguns. In Canada the response was similar, however not as fully extended as in the United States.In Canada today, the Canadian Air Transport Security Authority (CATSA) which is thoroughly explained in the next topic, handles all screening procedures by hiring third party private security firms to conduct screening. Our general airport security providing safety is executed by local Police forces. The RCMP once used to handle all general airport security, now only handles a few airports including Edmonton International Airport. Edmonton International Airport has a detachment of the RCMP located on site to ensure a quick and proper response to emergencies, ecurity and policing needs. The Police members on sit e are trained in emergency preparedness in order to be able to respond to a wide range of situations, including terrorism attacks. In addition to human Police officers, many airports such as Edmonton’s, implement canine members for certain situations such as sniffing out drugs, weapons, people, and explosives. In Edmonton specifically, at the RCMP airport detachment, they have Badge, a German Sheppard trained in attacking criminals, searching for lost people or baggage, as well as the ability to sniff out 15 different explosives.As well as the main goal of airport security of keeping a safe and problem free environment, security also includes Emergency Response Services (ERS). Fire and medical services are always ready at a moment’s notice 24 hours a day, 365 days a year for emergencies such as fires, aircraft mayday emergencies, and terrorist attacks. The Emergency Response Services are always highly trained and outfitted with top of the line vehicles and equipment to deal with any situation, and all have requirements and policies on stringent response times.An example of the preparedness by the ERS in Edmonton is their ability to make it from the fire hall located on site to the mid-point on the furthest runway in 3 minutes. Finally, as a direct result of the attacks of September 11, 2001 we seen the formation of the Canadian Air Transport Security Authority or â€Å"CATSA,† in December 2001, introduced by then Finance Minister Paul Martin, which included a very comprehensive plan aimed at enhancing economic and personal security in Canada.In April of 2002 CATSA took over responsibility of passenger and employee screening, although they still hire private security companies to do much of the screening, CATSA still oversees training and equipment available. CATSA states their mission is â€Å"to protect the public by securing critical elements of the air transportation system†¦ † Their responsibilities can be grouped into four major areas; Pre-board screening, hold baggage screening, non-passenger screening, and Restricted Area Identity Cards.Pre-board screening is done by Private Security firms hired and governed by CATSA, their tasks include getting all carry-on items coming onto an aircraft to go through X-ray, individuals must then pass through metal detectors and physical searches can and will be executed if an alarm is raised either through the metal detector, or random selection. Full body scanners are also used to reveal weapons, objects or explosives hidden under or within clothing. CATSA also implements the use of Explosive Detection Trace (EDT) technology at security checkpoints; this involves a quick swabbing of carry-on items or hands to test for explosives.Hold-Baggage Screening (HBS) is another major area of CATSA, this is what happens to our baggage once it has been checked in and is on its way to be loaded onto the aircraft. This is the process of screening all checked baggage using a co mbination of state-of-the-art explosive detection technology, as well as highly trained security personnel. During HBS the density of baggage is tested as well as additional testing for trace amounts of chemicals. HBS is currently in full deployment in 89 airports across Canada since 2006, for all domestic and international flights.CATSA is responsible for the instillation, deployment and renovations of HBS systems as well as regulatory compliance monitoring. Another major area of CATSA is non-passenger screening (NPS), this is required by Transport Canada starting back in 2004 and involves the random screening of people who work at the airports, these people can include flight crews, airport crews, caterers, maintenance workers, and baggage handlers. This subjects employees to unpredictable security screenings at entry points within airport restricted areas, the screening process is similar to that of the passenger screening, and also includes an Identification check.CATSA states t hat they screen 2,500 employees a day out of the 100,000 employees who have access to secure areas in Canadian airports. They also state that this program is continually evolving, as the only constant is â€Å"the cooperation needed between screening officers and non-passengers. † The fourth noted area of CATSA is the Restricted Area Identity Card (RAIC) program, which is constantly advancing through the use of biometric scanners. The RAIC program uses state-of-the-art duel biometric scanner identification programs to ensure the identity of the personnel who have restricted access.The program includes cards with built-in microchips to store biometric information of fingerprint and iris scanner templates. The RAIC program has been fully functional since January 31, 2007 in over 28 major airports in Canada. It’s notable that the biometric technology used here is also utilized in non-passenger screening and these two programs work in conjunction with one another. CATSA al so jointly implements the Canadian Air Carrier Protective Program (CACPP) with Transport Canada; this program is similar to that of the U.S. Sky Marshall Program. This program strategically places highly trained, armed, undercover RCMP members, also known as In-Flight Security Officers (IFSOs), on Canadian registered commercial airliners. IFSOs mandate is to be able to successfully prevent the aircraft from being taken over by an unauthorized individual(s), as well as gather and interpret intelligence on terrorist and criminal activity within civil aviation. In conclusion, airport security in North America and the world will continue to grow and prosper.In the post 9/11 world today, policies and procedures will only grow tighter and more stringent in an effort to prevent terrorism. From no-fly lists created to prevent the wrong people from boarding passenger planes, to tighter and better trained local Police and Security personnel on the front lines preforming screening, security an d emergency response services. To government initiatives like the Canadian Air Transport Security Authority providing important and well-rounded security systems from x-ray scanners to In-Flight Security Officers, the airline industry has never been more stringent in counter terrorism.References Airport screening: 9/11 response. (n. d. ). In CBC News; the fifth estate. Retrieved October 25, 2012, from http://www. cbc. ca/fifth/fastenseatbelts/security. html No fly list. (n. d. ). In Wikipedia. Retrieved October 25, 2012, from http://en. wikipedia. org/wiki/No_Fly_List Passenger Protect. (n. d. ). In Wikipedia. Retrieved October 25, 2012, from http://en. wikipedia. org/wiki/Passenger_Protect Security Screening. (n. d. ). In Canadian air transport security authority. Retrieved October 25, 2012, from http://www. atsa-acsta. gc. ca/Page. aspx? ID=7&pname=Screening_Controle&lang=en Canadian air carrier protective program. (n. d. ). In Transport Canada. Retrieved October 25, 2012, from ht tp://www. tc. gc. ca/eng/aviationsecurity/page-186. htm Gazette magazine – the canadian air carrier protective program . (n. d. ). In RCMP-GRC. Retrieved October 25, 2012, from http://www. rcmp-grc. gc. ca/gazette/vol70n3/air-eng. htm Emergency response. (n. d. ). In Fly EIA. Retrieved October 25, 2012, from http://www. flyeia. com/emergency_response. aspx

Particular population or society Essay

While people consider themselves unique individuals in terms of their personality, habits, opinions, attitudes personal quirks, there are many habits, beliefs and attitudes that are common to most people, especially those living a society. The culmination of language, religious beliefs, music, food preference, works habits, etc. of a particular population or society is known as its culture (Ember and Ember, 1993, p. 6). Culture is unique for every society or social group that sometimes what some would consider taboo like eating dog meat is actually part of another society’s customs. In theory, everyone should be open to other society’s culture but in reality, that does not happen often. An individual who gauges others culture is said to be an ethnocentric (p. 159). The attitude is termed ethnocentrism . This denotes that the person has difficulty criticizing his/ her own customs and traditions precisely because his/her vision is limited to his/her owns. Ethnocentrism does not only affect the individual’s view on other persons but influences the society as well. Watching a foreign film is one way to measure if one is open to other cultural experiences. I Not Stupid is a Singaporean film tackling the intertwined lives of three primary school classmates: Terry, Kok Pin and Boon Hock (Leong, Yin and Neo, 2002). It offers a glimpse of many facets of Singaporean culture: the education system that looks down on students in the bottom (the three are in EM3, which ranks lowest), the Chinese self-loathing (in a class, one character says she wants to be Caucasian when she grows up so she does not have to speak Chinese), the high regard for English (one mother speaks English to her children, another one stresses that in order to succeed in Singapore, one must be a master at English and Math), and Caucasian (a Caucasian was hired as director even though it was clearly evident that the local staff are more knowledgeable, equipped and deserving of the position; there is even a scene when the Singaporean boss approves the Caucasian’s concept even though it was the same concept presented by the locals, which he vehemently rejected), and the over obedience Singaporeans are known for (2002). The Singaporeans’ affair with Ba gua, or Chinese Pork Barbecue as well as their reliance on gods and goddesses to grant their favors is also shown in the film. Such rituals, as evinced in the film, may be common in Singapore but a North American, especially an ethnocentric, may find it peculiar or even distasteful. It must also be stressed that Singapore has a close relation with China so some of China’s cultural beliefs and customs are integrated into Singapore. The film is sprinkled in Chinese, English and Singlish, short for Singaporean English. Colonial mentality may be picked up from the film, judging the characters’ high regard for Westerners. The existence of Singlish is also a sign that Singaporeans, albeit already successful and one of the most economically driven countries in Asia, still feel the need to level on some ways with the Western countries, which this writer finds interesting. Imagine, in North America, there are students eager to learn other languages while in Singapore and other countries where there are other main languages aside from English, they already have the advantage of learning another language easily. Again, this is ethnocentrism at work. Another glaring cultural element that is shown in the film is Singaporean’s deference to power. Terry, one of the main characters, cannot stands on his own and always follows his mother. This is supposedly a jibe at Singaporeans’ compliant nature. If a similar event happened in North American, the kid is likely to fight back. But then again, this is Singapore and as said by one character, â€Å"It is difficult to catch fish in Singapore, because fish in Singapore are like Singaporeans, they’ll never open their mouths† (Leong, Yin and Neo, 2002). Ethnocentric thinking causes people to misunderstand other society’s culture and at the same it keeps them for understanding their own. The film I not Stupid is a satirical comedy carping on some of the country’s modern culture. Perhaps the producers are also ethnocentric but regardless of it, the film provides a peek at Singapore’s norms and values, which in essence is simply the country’s way of life. References Ember, C. and Ember, M. (1993). Anthropology 7th ed. New Jersey: Prentice Hall. Leong, D. and Yin, C. P. (Producers), and Neo, J. (Director). (2002). I not stupid [VCD]. Singapore: United International Pictures.

Civilization and Its Discontents Essay Example | Topics and Well Written Essays - 500 words

Civilization and Its Discontents - Essay Example The essay "Civilization and Its Discontents" talks about the central thesis of Sigmund Freud’s "Civilization and its Discontents" which is the assertion that the conflict between sexual needs and the societies restrictions is a big factor to human â€Å"dissatisfaction, aggression, hostility and ultimately, violence†. Given that the book was published in 1930, people’s mindsets were largely conservative, which led to protests and outcry against the book. But putting it in a purely scientific context, there is much truth in this thesis. Freud’s introduction to his book begins with the following lines: â€Å"Psycho-Analytic work has shown that these frustrations in respect of sexual life are especially unendurable to the so-called neurotics among us. These persons manufacture substitute-gratifications for themselves in their symptoms, which, however, are either painful in themselves or become the cause of suffering owing to the difficulties they create for the person's environment and society at large†¦ but culture demands other sacrifices besides that of sexual gratifications.† Further support for the Freudian view of human discontent could be found in Isabel Fonseca’s seminal work on the Gypsy tribes – Bury Me Standing. Fonseca attempts to decipher several aspects of the Gypsy way of life. The author shows that by virtue of remaining in their native geographies and not letting go of their primitive lifestyles, the Gypsies don’t live unfulfilled lives.

Variable Costing Essay Example | Topics and Well Written Essays - 1250 words

Variable Costing - Essay Example Product cost versus period cost Also known as inventorial costs, product costs are all those costs that are used in the acquisition and production of a product. They include direct labor costs, cost of direct materials and the manufacturing expenditure (Maher et, al 2006). They are normally assigned to an inventory account in the balance sheets and released from the stock as expenditure. Period costs are all the omissions in the product cost normally expensed on the income statement or report in the time of occurrence for example the office rent, administrative expenses and commissions on sale, which are expensed on the income statement during such periods of occurrence (Maher et, al 2006). ... The fixed manufacturing expenditure is incurred with the hope that the capital represented by these costs will be consumed in the manufacture of stock. Therefore these costs should correspond or rather be matched against the income attributed to the sale of such stock. Absorption cost needs the computation of expenditure rate for applying all the production expenses to the units that are produced during that period or else twin expenditure rates for variable manufacturing and fixed manufacturing overheads or multiple overhead rates in case the company uses the activity based costing (Hansen, et, al 2006). In variable costing, the validation is that the fixed manufacturing overhead shall be incurred in the short (er) term with no emphasis of the quantity of inventory that is produced. Even if the firm is not operating, a large portion of the fixed manufacturing overhead cannot be avoided in the short run hence the treatment of fixed manufacturing overhead as a period expanse. Though c onfusing in the sense that it implies only the direct manufacturing costs are capitalized, the fact is that the variable manufacturing overhead is also capitalized in equal measure. The name is far much less than ideal because not every variable cost is capitalized as the non manufacturing costs do not get to be capitalized as a portion of the stock or inventory in any circumstance. Usually, in variable costing, the cost of the ending stock is consisting of both the variable manufacturing overhead as well as the cost of direct manufacturing which is the materials and labor. Therefore, these are the costs for which the variable costing correspond the matching principle and as part of the inventory/stock, no other thing is

About german Essay Example | Topics and Well Written Essays - 250 words

About german - Essay Example The efficiency is admirable because if something is worth doing the Germans do it right. The obedience to the laws is also commendable. Laws are not created for citizens to pick and choose. They are for the betterment of society. Germans seem to understand this concept. As for the nudity when sunbathing, swimming, or in the sauna, this is laudable due to the shamelessness in the human body. Whether a svelte teenager or a wrinkled old woman the Germans are comfortable with their body. Americans are sometimes efficient, but not across the whole population. The efficiency personality varies among Americans. The obedience to the law is not as important to Americans. It seems that Americans pick and choose laws that are fair. If a law does not appear to be fair an American will fight to change it. For example at a red light in an emergency, an American will run the light. Germans would not. Americans enjoy nudity only for the good looking woman. Men can take off their shirts, but full nudity is obscene to Americans. Women can show everything but the chest and other private areas. Old people that are nude make the Americans disgusted. Germans and Americans are just different in these three

New Standards for Revenue Recognition Research Paper

New Standards for Revenue Recognition - Research Paper Example The IFRS stipulates minimum requirements on matters of revenue recognition. All in all the IAS 18 Revenue and IAS Construction Contracts are the main fields that it addresses. However, IAS 18 offers little explanation on issues that relate to multiple-element arrangements. The existing guidelines for revenue recognition as per the U.S. GAAP are so many. In the real sense, they cannot address the exact transactions or the target industries. With the mushrooming of new trends in transactions, the Board is facing challenges in addressing the issue. The first proposal was made on December 19, 2008 by both the IASB and the FASB. Bearing in mind that GAAP had a different approach from that of IFRS; the objectives made in the proposal were to make an improvement on the already existing guidelines. This gave birth to the concept of developing one model that can be utilized in addressing revenue recognition. In application, a company will be only able to recognize its revenue when the obligat ion is satisfactory. In this case, all services and transactions must satisfy their customer’s needs as per the contract (www.fasb.org, March 2014). On June 24, 2010, the Boards made a proposal to have the new joint standard for revenue recognition in place. In their view, adopting this proposal would bring the single revenue recognition standard in place. This will make it easy for IFRS and GAAP to address issues across all capital markets and industries with little differences (http://www.ifrs.org March, 2014).

History Essay Example | Topics and Well Written Essays - 1000 words - 18

History - Essay Example Death because the bubonic strain is typified by â€Å"large, inflamed lymph nodes around the neck, groin and armpits† that would turn black with the progression of the disease (Hayden, â€Å"History of The Black Death†). The spread of the disease was fast and crossed countries and continents within months as rat fleas feeding on infected black rats, causing the fleas to hunger for more sources of blood, were transported through ships that sailed from the East to the West (Hayden, â€Å"History of The Black Death†). Combined with the unsanitary conditions of the cities back then and contagion became inevitable. â€Å"The violence of this disease was such that the sick communicated it to the healthy who came near them, just as a fire catches anything dry or oily near it† (â€Å"The Black Death, 1348†). This was evidenced by black flags that were hung on villages and towns that were infected by the plague; almost everywhere these black flags were seen flying in the air (Butler, â€Å"The Black Death and its Impact (c.1300-1450)†). The rapid devastation of village and city populations created an aura of doom and fear—experiences that were never forgotten and gotten over with. Entire families died; survivors did not even have time to mourn their loved ones as the fear of contacting the disease was all-consuming (Holmes 249). People were forced to throw their loved ones in mass graves of ditches without a proper burial ceremony and even a hastened prayer (Holmes 249; James, â€Å"Black Death: The lasting impact†). â€Å"And there were those who had been so poorly covered with earth that dogs dragged them from there and through the city and fed on corpses† (qtd. in Holmes 249). Experiences like this are etched into the memory of the people and has inadvertently affected the psyche and morale of not only the individual, but of populations worldwide. This has resulted to a change in the way of living, especially for the peasants who were affected greatly as they did not have the

E-portfolios Essay Example | Topics and Well Written Essays - 1250 words

E-portfolios - Essay Example As results, the e-portfolio is termed as a repository that learners hold as proof for their development and a foundation to reflect on the learning process. It further encompasses education, delivery and receipt of comment, making plans and setting targets. In addition, collaboration and presentation to a group of people for a variety of reasons for instance partying, display of achievement and during recruitment into jobs. (Cambridge, 2012) States that a portfolio that is electronic consists of technologies that are electronic and gives the developer of the portfolio a variety of formats to organize the artifacts. E-portfolios are complex with functionalities like an administration tool to run and present work build with a number of applications and to establish who has the privilege to see the work. In general e-portfolios enable learners to gather various works and present their portfolios in varied formats. That is inline with the understanding that knowledge dwells in the perspe ctive of both the past and current relationship socially. Teachers traverse five levels in the process of using E-portfolios to weigh their learners. The first stage includes identification of intent, the standards, and addressees. An illustration is the basis of the outcome of the students target by the teacher like establishing cohesion and coherence in essay writing. Teachers then create a means to evaluate the e-portfolio like a framework or behaviors that are observable. Next involves determining the category of the audience as teachers, parents or professors because these addressees affect the designing of the e-portfolio. The following is the selection of the location for the storage, choosing the software tool and assembling artifacts meeting the standards. For proper utilization of the e-portfolio, make a suitable choice of the software. As a result, the consideration by teachers is suitability and availability of software

International Monetary Fund Essay Example | Topics and Well Written Essays - 1000 words

International Monetary Fund - Essay Example The International Monetary Fund (IMF) is an inter-governmental organization which was established to endorse international money cooperation, systematic exchange arrangements and exchange stability. The International Monetary Fund (IMF) offers the technical and financial assistance to its members in different areas of economic policy–basically in the field of exchange rates, fiscal, monetary and financial sector policies (The World Economy, â€Å"The International Monetary Fund†). Overview of the International Monetary Fund (IMF) The International Monetary Fund (IMF) was established in July 1944, under the ‘Bretton Woods System’ which comprised of three international organizations, those were: The International Monetary Fund (IMF): It was established with a purpose of developing International Monetary Cooperation. The International Bank of Reconstruction and Development: This was established with a motive of International Development Assistance and Investme nt. The International Trade Organization: It was established with a motive to develop International Trade. The above three components of The Bretton Woods System were entertained in the context of war, high unemployment, depression, hyper inflation and high fluctuating exchange rates which effected the global economy in the 1930s (Sykes, â€Å"Organisational Information†). ... Surveillance Consultations: Consultations concluded for 120 countries in FY 2010 and for 88 countries in FY 2011 as on 11/02/2011 (The International Monetary Fund, â€Å"The IMF at a Glance†). The IMF’s governing body essentially, is the board of governors. Each state is being represented by their respective elected governor (member of the Board of Governor, IMF). The IMF’s executive board is its executive body, and is comprised of 24 Executive Directors; each of them is either elected or appointed by the members. The executive board of the IMF is responsible for regulating the smooth flow of business of the organization. There are two committees, the International Monetary and Financial Committee and the Development Committee which are appointed by the Board of Governors (Sykes, â€Å"Organisational Information†). Merits and De–Merits The IMF has executed many reforms till date, it has been designed to strengthen its network and improve its capabil ity to perform well and serve its membership efficiently. The IMF has rapidly become an open and transparent organization; it has also inculcated transparency among its membership. Moreover, it is also working to strengthen its economic governance. These aspects have been undertaken through promoting the use of codes and standards as a medium for better financial management, economic and corporate governance. The IMF is also working to safeguard the integrity and stability of the International Financial System globally. More specifically, the IMF, the World Bank along with the Financial Sector Assessment Program (FSAP) perform together to strengthen the combat money laundering sectors along with the financial sectors in member countries. The International Monetary Fund (IMF) is an

Male Intimacy Friendship Article Example | Topics and Well Written Essays - 500 words

Male Intimacy Friendship - Article Example Studies conducted recently have shown that men usually do not have same-sex friendships that are satisfying as the ones of the women. Michael McGill adds to this by saying that to say men possess no intimate friends appear too harsh on the surface, but the data indicate that it is almost the truth†¦their relationships with the other men are superficial even shallow. It has been noted that intimacy is absent in the connection of friendships found among men and they are dissatisfied with these types of friendships. This article explores the intimacy concept particularly among the adult male friendships by examining comradeship, which is a form of male friendship. Strikwerda further gives an account of both the nature and value of intimacy and the obstacles to it followed by a focus on the intimacy process. Male friendships are compared to the friendship of young children engaging in a parallel play. The individuals do not really interact but merely play at a side-by-side distance. This type of companionship is very enjoyable but lacks intimacy. This friendship is not impoverished, but the truth is that if more is invested in it then it will lead to hunger for something more.aring of given kinds of experiences examples being the ones during those of soldiers in trenches, teenage boy, and sailors on a voyage, and this type of friendship provides a mutual self-disclosure occasion among the males. A characteristic that is highlighted to play a vital role in comradeship is loyalty. The war situation depicts the comrades’ loyalty as being abstract and not personal terms. Gray is quoted to say that comrades become loyal to each other in a spontaneous manner, and with no need for the reasons. He further argues that the loyalty, however, is fragile because it is not connected necessarily to the feeling of one belonging and spontaneous liking.

Qualitative Research Project Essay Example for Free

Qualitative Research Project Essay

Dextranase Enzyme Production

Dextranase Enzyme Production Dextranase definition and its uses Dextran is a collective name given to a large class of homopolysaccharides composed of D-glucans with contiguous a-1, 6 glycosidic linkages (95%), with minor secondary linkages such as a-1, 2, a-1, 3 and a-1, 4 [74]. It is produced by microorganisms such as Leuconostoc mesenteroides, Streptococcus sp., Acetobacter capsulatus and Acetobacter viscus [44]. Dextrans are well soluble in water, have low toxicity, and relative inertness. These properties make dextrans effective water-soluble carriers for dyes, indicators, and reactive groups in a wide variety of applications. They are widely used in the pharmaceutical and biochemical fields. Dextrans of low molecular weight are used as an alternative to blood plasma. They are also used for clinical purposes such as drug delivery [82], and by cross-linking for the production of the chromatographic matrix Sephadex. They are also widely used as both anterograde and retrograde tracers in neurons [94]. On the other hand microbial synthesis of de xtrans in damaged cane and beets or other products containing sucrose is a serious problem in sugar and food industry. Dextran is also a structural component of dental plaque which causes the development of dental caries [78], [85]. Dextranases are enzymes that cleave the a-1,6 glycosidic linkages of dextran to yield either glucose or isomaltose (exodextranases) or isomalto-oligosaccharides (endodextranases), and are only produced as extracellular enzymes by a small number of bacteria and fungi, including yeasts and perhaps some higher eukaryotes [44]. Enzymes in many groups can be classified as dextranases according to function: dextranhydrolases, glucodextranases, exoisomaltohydrolases, exoisomaltotriohydrases, and branched-dextran exo-1,2-alpha glucosidases. In particular the chemical reaction catalyzed is as follows: (1,4-alpha-D-glucosyl)n + (1,4-alpha-D-glucosyl)m à ¢Ã¢â‚¬  Ã¢â‚¬  (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m + 1 These enzymes belong to the family of glycosyltransferases, specifically the exosyltransferases. The systematic name of this enzyme class is: 1,4-alpha-D-glucan: 1,6-alphaD-glucan 6 alpha-d-glucosyltransferase. Other commonly used names include dextrin 6-glucosyltransferase and dextrin dextranase. Many microorganisms are known to produce dextranase, including filamentous fungi belonging to the genera Penicillium, Aspergillus, Spicaria, Fusarium and Chaetomium, bacteria, e.g. Lactobacillus, Cellvibrio, Flavobacterium etc. The only yeasts reported to produce dextranases are members of the family Lipomycetaceae. Only Lipomyces kononenkoae [104] and Lipomyces starkeyi dextranases have been characterized [47]. Potential commercial uses of dextranases include: The synthesis of potentially valuable oligosaccharides [30] Potential mouthwash ingredients since isomaltose may be of significant importance for the prevention of dental caries [40], [41] Clearance of dextran contamination in cane sugar processing [25] Dual-stimuli-responsive drug release as in biodegradable polymer-structured hydrogels of gelatin and dextran [55]. Hydrogels are used for a wide range of biomaterials applications such as: contact lenses, drug delivery vehicles and tissue adhesives. Dextrans are polymers that mimic biological sugars found on tissue surfaces. The dextran hydrogel system with tunable mechanical and biochemical properties appears promising for applications in cell culture and tissue engineering [58] Drug delivery device suitable for delivering drug to the colon [7], [8]. Brondsted et al. studied the application glutaraldehyde dextran as a capsule material for colon-specific drug delivery. The dextran capsules were challenged with a dextranase solution, simulating the arrival of the drug delivery to the colon, so they broke and the drug was released as a dose pump. The outcome highlights the dextran capsules as promising candidates for providing a colon-specific drug delivery Also in site-specific drug delivery systems with the use of antibodies [69] The improvement of brewing yeast strain for beer industry. Due to the rising demand for low-calorie beverages, including beer, recombinant strains of Saccharomyces cerevisiae have been produced by integrating LSD1 gene of Lipomyces starkeyi [101]. S. cerevisiae lacks the ability to produce extracellular depolymerising enzymes that can efficiently liberate fermentable sugar from abundant, polysaccharide rich substrates [75]. By introducing the gene mentioned above, adding an exogenous enzyme during beer fermentation to achieve starch hydrolysis and oligosaccharide reduction can be avoided Carbohydrase activity produced can also be exploited in sensitive chromogenic bioassays for toxicity: a mycotoxin bioassay using the intracellular ÃŽÂ ²-galactosidase activity of Kluyveromyces marxianus has been developed [20] Classification of dextranase based on amino acid sequence Dextranases are dextran-degrading enzymes that form a diverse group of carbohydrases and transferases. The more recent classification divides dextranases into two classes: endodextranases (a-1,6-glucan-6-glucnohydrolase; also referred to as dextranase) and exodextranases ( glucan-1,6-ÃŽÂ ±-glycosidase; also referred to as dextran glucosidases). The Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUB-MB) provides a system of classification depending on the type of reaction catalyzed and product specificity (Table 1). Contrary to that system, the Carbohydrate Active Enzymes (CAZy) database describes the families on terms of structural and mechanical features of these enzymes; enzymes with different substrate specificities are placed in the same family and those that hydrolyze the same substrate are sometimes placed in different families. In another classification system, Henrissat and Bairoch [33] have divided glucosylhydrolases and glycosylt ransferases into five families on the basis of the similarities in the amino acid sequences (Table 2). Table 1: The IUB-MB classification system EC3.2.1.11 Dextranases EC3.2.1.70 Glucan-1,6-glucosidases EC3.2.1.94 Glucan-1,6-isomaltosidases EC3.2.1.95 Dextran-1,6-isomaltotriosidases EC3.2.1.115 Branched-dextran exo-1,2-glucosidases Table 2: Classification of dextran hydrolysing enzymes, based on amino acid sequences. Dextran-glucosidases Families 13 15 Isomaltodextranase Family 27 Isomaltotriosidase Family 49 Endodextranases Families 49 66 (no sequence similarities between the two families) Aoki and Sakano (1997) came up with 4 families [2]. They isolated and sequenced the isopullunase gene (ipuA) from Aspergillus niger ATCC 9642. The gene shows significant amino acid similarity to the dextranase produced by Penicillium minioluteum (PEMDEX) and Arthrobacter sp. (ARTDEX). Since ASNIPU shows great similarity to PEMDEX and ARTDEX, they can be classified as Family 1. In the same fashion, the researchers compared the amino acid sequences of dextranases and dextran-hydrolising enzymes, including ASNIPU. Lipomyces species and Lipomyces starkeyi Lipomyces starkeyi and Lipomyces kononenkoae belong to the Lipomycetaceae family and are the only yeasts reported to produce dextranases. The first Lipomyces species was identified by Robert Starkeyi in 1946 during a study of nitrogen-fixing bacteria: it was then that he discovered L. starkeyi, a fat-producing, ascosporogenous soil yeast. The family Lipomycetaceae was proposed later, in 1952 by Lodder and Kreger von Rij. Lipomyces species can utilize starch as a sole source of carbon. Both species contain highly efficient amylolytic systems, permitting growth on starch with very high biomass yields [97]. The family Lipomycetaceae is known to utilize certain heterocyclic compounds, such as imidazole, pyrimidine, and pyrazine and their derivatives, as sole nitrogen sources [92]. Information on the genome organization and molecular genetics of this group of yeasts is very limited. The ascosporogenous soil yeast L. starkeyi has been reported to produce commercially useful extracellular dextranase activity [97], [52], [53], and it can utilize a variety of other compounds, like hexoses, pentoses, alcohols and organic acids, as sole sources of carbon and energy [46]. The strains of L. starkeyi currently used are NCYC 1436, IGC 4047, ATCC 12659 and its de-repressed mutant ATCC 20825. L. starkeyi dextranases Commercial use of dextranase began in 1940s, mainly by producing low-molecular-weight clinical dextran. Therefore, industrially practical mixed culture fermentation of L. starkeyi and Leuconostoc mesenteroides was capable of producing controlled-size dextrans in order to satisfy clinical use, in which dextranase produced by L. starkeyi hydrolyzed the high molecular weight dextran produced by L. mesenteroides to a controlled size [46]. The enzyme production system of L. starkeyi needs an inducer. Dextran is its normal inducer but it is a relatively expensive carbon source for large-scale fermentations. Also, L. starkeyi is reported to have slow growth and difficulty of avoiding contamination from other microorganisms during growth. With that in mind D. W. Koenig and D. F. Day (1989) undertook to establish conditions which would minimize the cost of the inducer for producing an enzyme by using a de-repressed mutant of L. starkeyi ATCC 12659 grown on glucose. Thus the mutant ATCC 20825 is capable of hyperproducing dextranase at low pH to provide biologically contaminant-free supernatant liquid containing dextranase. Lipomyces starkeyi (IGC 4047), when grown on dextran as a sole carbon source produced a dextranase able to hydrolyse blue dextran and Sephadex G-100. The molecular weight was 23kDa and the isoelectric point was 5.4 [97]. The dextranase of L. starkeyi (ATCC 20825) studied by Koening and Day (1988, 1989a, 1989b) was analysed by SDS-PAGE and produced four bands, of molecular weights 65 kDa, 68 kDa, 71 kDa, and 78 kDa. Millson and Evans (2007) have isolated extracellular dextranase of L. starkeyi NCYC 1436 and have found that for their strain the enzyme occurs as three molecular weight species and seven isoelectric forms [68]. L. starkeyi nutrients (YPDex / YPD) The main ingredient in the chosen media is yeast extract. Yeast extract is a dried autolysate which facilitates rapid and luxuriant growth when used in various media or fermentation broth. It is a good source of amino-nitrogen and vitamins, especially the water-soluble B-complex vitamins. However, yeast extract is reported to enhance glucose metabolism to lipids, but inhibit lipolysis [18]. The metabolic pathway consists of converting glycerol into pyruvate or glucose and then hydrolysis by a phosphatase gives glycerol again. The disruption of this metabolic pathway, could account for the seemingly truncated numerous bands that SDS gives after prolonged storage of the yeast. Mycological peptone is incorporated in the media and discourages bacterial growth because of its acidity. Environment that dextranases favour Dextranase activity is affected by temperature, pH, metal ions and nutrients. According to Lin Chen et al (2007), dextranase activity is optimized between temperatures of 10oC and 60oC at pH of 6.0 [12]. In the particular study, the effect of pH on enzyme activity was determined by varying the pH between 3.5 and 8.5 under the temperature of 30oC. The pH of 3.4-4.5, 5.0-7.5, and 8.0-8.5 were maintained by sodium acetate buffer (20mM), citrate and phosphate buffer (20mM) and sodium phosphate buffer (20mM) respectively. The effects of metal ions (AlCl3, CaCl 2, CoCl2, CuSO4, FeCl3, KCl, MgCl2, NaCl, NiSO4, MnCl2 and ZnCl2) and SDS on dextranase activity were assayed by incubation of dextranase with 1mM metal ions or 1 mM SDS at pH 4.5 for 3h at 37oC, and then the enzyme activity of dextranase was determined. Ravi Kiran Purama and Arun Goyal (2008) in a study for optimization of nutritional factors, estimated dextransucrase activity in the cell free extract of Leuconostoc mesenteroides. They analysed the regression coefficients and t-values of six ingredients: yeast extract, sucrose, intercept, K2HPO4, beef extract, peptone and Tween 80. Yeast extract, sucrose, beef extract, and K2HPO4 displayed a positive effect for enzyme production whereas, peptone and Tween 80 had a negative effect on enzyme production. The variables with confidence levels greater than 90% were considered as significant. Sucrose was significant at 99.99% confidence levels for dextransucrase production. K2HPO4 and yeast extract were found significant about 94% level for dextransucrase production. Beef extract was significant 91% for dextransucrase production. Peptone and Tween 80 were found insignificant with negative coeffficients for enzyme activities. Methods used for enzyme activity measurement Enzymatic activity is measured with the help of laboratory methods called enzyme assays. All enzyme assays measure either the consumption or production of product over time. Enzyme assays can be split into two groups according to their sampling method: continuous assays, where the assay gives a continuous reading of activity, and discontinuous assays, where samples are taken, the reaction stopped and then the concentration of substrates/products determined [11], [20]. Continuous assays: Spectrophotometry in which you follow the course of the reaction by measuring a change in how much light the assay solution absorbs Fluorimetric assay in which we make use of the difference in the fluorescence of substrate from product to measure enzyme reaction. These assays are in general much more sensitive than spectrophotometric assays, but can suffer from interference caused by impurities and the instability of many fluorescent compounds when exposed to light Calorimetric assay in which the heat released or absorbed by chemical reactions is measured Chemiluminescence in which the light emitted by some enzyme reactions is measured so as to detect product formation. The detection of horseradish peroxidase by ECL is a common method of detecting antibodies in western blotting Discontinuous assays: Radiometry in which the incorporation of radioactivity in substrates is measured Chromatographic assays measuring product formation by separating the reaction mixture into its components. This is usually done by high-performance liquid chromatography (HPLC), but thin layer chromatography can also be used. Although this approach needs a lot of consumables its sensitivity can be increased by labelling the substrates/products with a radioactive or fluorescent tag Methods and assays for dextranase activity measurement The large variability of available substrates makes it difficult to estimate the enzyme activity, because the reaction product is often an undefined mixture of sugar polymers. The existing assays try to compromise convenience, speed and accuracy [44] Viscosimetric analysis was among the first to be used [31], [35], [36]. This method measured the amount of enzyme which reduced the specific viscosity of the dextran solution by half in 10min. and it is more suitable when dextranase hydrolyses the dextran molecule at random, producing long oligosaccharides. Reducing-sugar assay or saccharogenic methods measure the rate of increase in reducing sugar as measured with the Somogyi assay, the 3,5-dinitrosalicylicacid method (DNS) [102], thiourea borax-modified O-toluidine colour reagent (35) and alkaline potassium ferricyanide solution (225). These methods test the presence of free carbonyl group (C=O). It is a simple method commonly used to analyze for reducing sugars produced from enzymatic hydrolysis of substrates such as starch and sucrose [67].The most common substrates applied are Dextran T2000,47 T-260,3 and T110 [54], [72]. A number of substances have been reported as interfering with DNS colour development and citrate is one of them. Acetate and citrate are reported to enhance colour development and the true antagonist in this reaction is the proton (H+) [96]. This method is based on the release of short coloured products from polymeric blue dextran and their selective colorimetric detection at 610-650nm after precipitation of the p olymer. DNS colorimetric assays reported in literature are often modifications of the method of Webb and Spender-Martins (1983). E. F. Khalikova and N. G. Usanov (2001) developed a dextranase assay using an isoluble substrate, namely, Sephadex G-200 with Remazol Brilliant Blue dye [45]. The action pattern of dextranase was then, studied by means of exclusion chromatography. Overall, this assay was reported as convenient for quantitative dextranase detection, relatively independent of the enzyme source, and is proposed as an inexpensive alternative to the known procedures utilizing coloured substrates. The dextranase substrates can be either dye-releasing or fluorogenic. The assay procedures based on these substrates are accurate, fast and can be recommended for dextranase-producing microbial screening and enzyme purification. Other assay procedures worth mentioning include a spectrophotometric method with the use of Blue Dextran developed by Kauko K. Makinen and Illika K. Paunio (2004) who recommend it for column chromatography [62], and a method based on simple titration, developed by Eggleston and Gillian (2005) for easy use at the sugar cane factory [19]. Fluorometric assays are based on measuring the fluorescence of the samples and the results are often compared to a series of standards of Penicillium sp. A very sensitive fluorometric assay using amino-dextran-70 coupled with fluorescent dye BODIPY (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester) as the substrate was described by M. Zhou et al. (1998). The BODIPY FL dye-labelled dextranase substrate is an amine-containing dextran derivative that is labelled with the pH-insensitive, green fluorescent BODIPY FL dye, resulting in almost total quenching of the conjugates fluorescence. The increase of the fluorescent degradation products of BODIPY FL dextran is proportional to the amount of dextranase activity [102]. A suspension of Sephadex in a buffer is supplemented with agar, sterilized, and poured in Petri dishes, and after the wells are filled with the test solution, they are left to incubate. The dextranase activity can be evaluated by the extent of halos around the holes due to the opalescence of Sephadex. Milson and Evans (2007), measured dextranase activity using SDS PAGE as described by Laemmli (1970), using both mini-gel and Protean II electrophoresis systems, and stained using Coomassie Blue [68], [56]. Molecular weight markers were used to construct a calibration curve, from which molecular weights of dextranase were determined. Native gel electrophoresis was performed, but the loading buffer and the gel lacked SDS and ÃŽÂ ²-mercaptoethanol and the samples were not heated prior to loading on the gel. In the same study, dextranase activity was estimated in SDS gels, without extraction, by a plate modified from the method of Lawman and Bleiweis (1991) [57]. FL versus DNS assay method The classic method (DNS) for measuring glycosidases through release of reducing activity is simple and inexpensive and, as cited above, has been modified in several studies so as to suit the researchers needs. It may, however, have some pitfalls. The reaction taking place is the following: aldheyde group oxidation> carboxyl group 3,5-dinitrisalicylic acid reduction-> 3-amino,5-nitrosalycilic acid (Nam Sun Wang, University of Maryland) The above reaction scheme shows that 1 mole of sugar reacts with 1 mole of 3,5-dinitrisalicylic acid. However, it is suspected that there are many side reactions, and the actual stoichiometry is more complicated than that previously described. Different reduced sugars yield different colour intensities; thus it is necessary to calibrate for each sugar. Apart from the oxidation, other side reactions may compete for the availability of 3,5-dinitrisalicylic acid. Consequently, the calibration curve may be affected and the intensity of the developed colour may be enhanced. Therefore, the method has low specificity and one must run blanks diligently if the colorimetric results are to be interpreted correctly and accurately [96]. Another obstacle to be dealt with when using DNS is non-linearity. One cause of non-linearity could be the common practice of diluting reaction products before quantification of reducing compounds and another is the insufficiency of substrates. The fluorometric assay (FL), seems to gain ground in the most recent studies as faster and more accurate and it seems to leave space for modifications and combined use with other methods (see  §1.3.1). A standard curve is constructed from Penicillium sp. and then compared with the one derived from Lipomyces starkeyi. As described in the previous paragraph dextranase activity is estimated by the increase of the fluorescent products of dextran degradation. However, if too many fluoro are conjugated to the dextran molecule undesired may come up. Molecular Probes TM seems to overcome this problem by removing as much of the free dye as possible and then assaying the fluorescent dextran by (TLC) to ensure that it is free of low molecular weight dyes. So, in general, FL seems to yield accurate curves. Millson and Evans (2007), used an assay of dextranase activity which was a variation on that reported by Zhou et al. (1998). In that study, fluorescence vs. dextranase activity produced a linear log [68], [102]. Purification of L. starkeyi dextranase Dialysis tubing Dialysis tubing is typically used for changing the buffering solution of a protein and is also a method for concentrating protein solutions by dialysis against a hygroscopic environment (e.g. PEG, Sephadex). The protein solution is contained within a membrane which permits solute exchange with a surrounding solution and whose pore size prevents the protein from escaping. Except for small volumes, this method is time-consuming [11]. Filtration Ultrafiltration Ultrafiltration (UF) is a variety of membrane filtration in which hydrostatic pressure forces a liquid against a semi-permeable membrane. Suspended solids and solutes of high molecular weight are retained, while low molecular weight solutes pass through the membrane. UF is not fundamentally different from microfiltration or nanofiltration, except in terms of the size of the molecules it retains. [11], [77]. SDS-PAGE Purification of Lipomyces starkeyi dextranase is carried out mainly by running a SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) analysis. The solution of proteins to be analyzed is first mixed with SDS, an anionic detergent which denatures secondary and non-disulfide-linked tertiary structures, and applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4g SDS per 1.0g protein. The size of the protein is directly related to the distance it migrates through the gel. Dextranase molecules migrate as bands based on size. Each band can be detected using stains such as Coomassie blue dye [77]. Modifications to the polypeptide backbone, such as N- or O- linked glycolylisation, however have a significant impact on the apparent molecular weight. Thus, the apparent molecular weight is not a true reflection of the mass of the polypeptide chain. In most cases, SDS-polyacrylamide gel electrophoresis is carried out with a discontinuous buffer system in which the buffer in the reservoirs is of a different pH and ionic strength from the buffer used to cast the gel. After migrating through a stacking gel of high porosity the SDS-polypeptide complexes are deposited in a very thin zone (or stack) on the surface of the resolving gel. The discontinuous buffer system that is most widely used was originally devised by Orstein (1964) and Dvis (1964) [77]. The sample and the stacking gel contain Tris Cl (pH 6.8), the upper and lower buffer reservoirs contain Tris-glycine (pH 8.3) and the resolving gel contains Tris Cl (pH 8.8). All components of the system contain 0.1% SDS [56]. Precipitation methods of proteins Precipitation is widely used in downstream processing of biological products, especially proteins. It serves to concentrate and fractionate the target product from various contaminants, as in biotechnology industry where precipitation helps to eliminate contaminants commonly contained in blood. The underlying mechanism of precipitation is to alter the solvation potential of the solvent and thus lower the solubility of the solute by addition of a reagent. Precipitation is usually induced by any of the following methods [11]: Salting out Isoelectric point precipitation Precipitation with organic solvents Non-ionic hydrophilic polymers Flocculation by polyelectrolytes Polyvalent metallic ions Salting out This the most common type of precipitation. Normally a neutral salt is added, such as ammonium sulphate, which compresses the solvation layer and increases protein protein interactions. As the salt concentration of a solution is increased, more of the bulk water is associated with the ions. Consequently, less water is available to partake in the solvation layer around the protein, which exposes hydrophobic interactions, aggregate and precipitate from solution. Isoelectric point precipitation The isoelectric point (pI) is the pH of a solution at which the net primary charge of a protein becomes zero. At a solution pH that is above the pÃŽâ„ ¢ the surface of the protein is primarily negatively charged and therefore like-charged molecules will exhibit repulsive forces. At a solution pH that is below the pI, the surface of the protein is primarily positively charged and repulsion between proteins occurs. At the pI, the negative and positive charges cancel, repulsive electrostatic forces are reduced and the dispersive forces predominate, and will, therefore, cause aggregation and precipitation. The pI of most proteins lies in the pH range of 4-6. Mineral acids, such as hydrochloric and sulphuric acid are used as precipitants. The greatest disadvantage to isoelectric point precipitation is the irreversible denaturation caused by the mineral acids. For this reason isoelectric point precipitation is most often used to precipitate contaminant proteins, rather than target pro tein. Precipitation with organic solvents Ethanol or methanol, if added to a solution may cause the proteins of the solution to precipitate. As the organic solvent gradually displaces water from the surface of the protein and binds it in layers around the organic solvent molecules, the solvation layer around the protein decreases. In that state, the protein can aggregate by attractive electrostatic and dipole forces. Parameters to consider are temperature (should be less than 0 °C to avoid denaturation), pH and protein concentration of the solution. Miscible organic solvents decrease the dielectric constant of water, which in effect allows two proteins to come together. At the pI the relationship between the dielectric constant and protein solubility is given by: log S = k/e2 + log S0 S0 is an extrapolated value of S, e is the dielectric constant of the mixture and k is a constant that relates to the dielectric constant of water [98]. Non- ionic hydrophilic polymers Dextrans, polyethylene glycols and other polymers are used in precipitation of proteins due to their low flammability and are less likely to denature biomaterials compared to pI precipitation. These polymers attract water molecules away from the salvation layer around the protein, which enforces protein-protein interactions and induces precipitation. For the case of polyethylene glycol, the following equation models precipitation: ln(S) +pS = X ÃŽÂ ±C C is the polymer concentration, P is a protein-protein interaction coefficient, ÃŽÂ ± is protein- polymer interaction coefficient and X = ( ÃŽÂ ¼i ÃŽÂ ¼i0 )RT ÃŽÂ ¼ is the chemical potential of component I, R is the universal gas constant and T is the absolute temperature [98]. Flocculation by polyelectrolytes Polyelectrolytes form extended networks between protein molecules in solution. These include alginate, carboxylmethylcellulose, polyacrylic acid, tannic acid and polyphosphates. The pH of the solution determines the effectiveness of these polyelectrolytes. Anionic polyelectrolytes are used at pH above the pI. Cationic polyelectrolytes are used at pH above the pI. The precipitate may dissolve back into the solution if an excess of polyelectrolytes is used. Polyvalent metallic ions Enzymes and nucleic acids are precipitated with the use of metal salts at low concentrations. Most frequently polyvalent metallic ions used are Ca+, Mg+, Mn+ or Fe+. Precipitation reactors Industrial scaled reactors that are used to precipitate large amounts of proteins, such as recombinant DNA polymerases from a solution include: Batch reactors The agent is slowly added to the protein solution under mixing, so the aggregating particles tend to be regular in shape. The protein particles are exposed to a wide range of shear stresses for a log period of time and become mechanically stable. Tubular reactors The precipitating reagent and the feed protein solution are contacted in an area of mixing and then added into enlongeted tubes where precipitation occurs. Plug flow is approached by the elements as they move along the tubes. The tubular reactor is inexpensive to be constructed but can become long and slow in case that aggregation of the particles occur slowly. Continuous stirred tank reactors CSTR reactors also known as vat or back mix reactors, run at steady state with a continuous flow of reactants and products in a well-mixed tank. It is a type of reactor mainly used in chemical engineering. A CSTR often refers to a mathematical model which is used to estimate the key unit operation variables when using a continuous agitated-tank reactor to reach a specified output. Perfect mixing is demanded. Precipitation of L. starkeyi The most common precipitation methods in the case of L. starkeyi cited in literature are: Isoelectric focusing Koening and Day (1988) used precast IsoGel agarose isoelectric focusing plates, pH 5.0-8.5. A standard mixture of proteins was applied in the lane next to each sample and the protein profile was quantified by densitometer scans. The enzyme activity in the gel was determined by slicing an unstained gel into 0.9 mm sections. Each section was placrd in a test tube with 1.0 ml 0.05 M citrate/phosphate (pH 5.5) buffer, allowed to elute overnight at 4oC and assayed for enzyme activity. This method separated the protein mixture into five isoelectric bands. All five forms were found to have dextranase activity and exhibited the same Km values. Organic solvents Polyethylene glycol precipitation is often used. Nishimura et al. (2002) used this method in an effort to prepare total DNA from L. starkeyi for taxonomy analysis. They added phenol solution (phenol: chloroform: isoamyl alcohol=25:24:1) to a test tube of Tris-SDS. The

STRATEGIC MANAGEMENT AND LEADERSHIP SKILLS INDUS TOWERS LIMITED

STRATEGIC MANAGEMENT AND LEADERSHIP SKILLS INDUS TOWERS LIMITED Strategic  leadership  basically means using strategy in the  management  of workers. The main strategy usually employed in a strategic style of  leadership  is to motivate workers to take the initiative to improve their productive input into the company. Strategy involves thinking and planning.  Leadership  inspires others to take the appropriate action. Strategic  leadership  is a management  model that trains and encourages employees to best prepare the company for the future.Strategic leaders are always looking ahead and analyzing the present in terms of preparation for what may be ahead for the business. Awareness is a big part of a strategic  leadership  style, but it must be followed up with well thought out action. Strategic leaders are adaptable and growth-oriented. They take responsibility for getting things done by training employees to think and act more effectively to achieve the best result possible for the company. 1.2. The Impact of Management and Leadership Styles on Strategic Decisions Company Background Aircel group is a mobile phone service provider in india. It offers both prepaid and postpaid GSM Cellular phone coverage throughout India. Aircel is a joint venture between Maxis Communication of Malaysia and Apollo Hospital Enterprise Ltd of India. UTSB has a 74% stake in Aircel and the remaining 26% is with Apollo Hospitals. It is Indias Seventh largest GSM mobile service provider with a subscriber base of over 51.83 million, as of January 31, 2011. It has a market share of 6.72% among the GSM operators in the country. As on date, Aircel is present in all 23 telecom circles. Aircel placed an actual dinghy lifeboat to a downtown billboard. A rope with a sign reading, In case of emergency, cut rope, held up the branded raft. July 15, 2009 the monsoon arrived with flooded streets and so did Aircel customer service. The dinghy was cut down and pedestrians were safely transported. What Aircel calls Corporate Social Responsibility A Solution. The company was able to generate positive p ublicity and show consumers that they care. Aircel is famous for innovative Pocket Internet cards for Free GPRS service for 1day/3days/7days/30days. Aircel become first to introduce Online service to subscribe and manage Dialer tunes. It is first in country to introduce SMS bank/Phone Book/Reminder/Talking SMS. In the past several decades, management experts have undergone a revolution in how they define leadership and what their attitudes are toward it. They have gone from a very classical autocratic approach to a very creative, participative approach. Somewhere along the line, it was determined that not everything old was bad and not everything new was good. Rather, different styles were needed for different situations and each leader needed to know when to exhibit a particular approach. 1.3.Leadership Styles Can Be Adopted For Different Situations A leadership style survey is a innovative thinking of starting about leadership. using leadership style in organization is a different way from leadership competencies of explaining leadership. Leadership style is the essential quality in the organization, implementing plans, innovative thinking, initiative, motivating the team members Three of the most basic leadership styles are: Autocratic Laissez-faire Democratic Autocratic: This is something one peson decision, the leader tells the employees what to do and what not to do, the employees dont have any option they have to follow their leader instructions. The employees dont have the liberal freedom by thinking of innovative or they cant take any initiatives in the work or growth for the organization. This style can be followed only if the employees have all the sources in the hand, because its something the leader orders to the employees and they follow them, so if every information is available to the employees they can finish the work with in the time frame of the limit. Actually this type of style should not be used as it is a emergency case. If we need initiatives and commitment from our employees we have to motivate our employees, so we have to follow the participative style. In this autocratic style we have a gap leaders and employees this leads to improper communication and understanding. Laissez Faire: In this style the leader gives the full liberty to the employees to take there own decision. But its not something final what the employee does, the employee has every rights to take the decision, However leader is responsible for whatever the decision is been made, The decision can be made where the employee should know about the in and out of the process and analyse the situation how the decision can be made, we cant make the blind decision, we can set the target to complete the task. This is not a style we can blame the employees if things went wrong, its something we are trusting employees and having confidence of the people who work below us. We should not afraid of using this style, we can use it wisely, by following this style employees will gain confidence and they can grow as an future leader. Democratic: This is the current style used in the organization, this style leader and employee merge together and comes to the final decision. Because certain things can be assessed only by the employees and not by the leader. The work done by the employees cannot be predicted how to handle this issue in the hectic situation. So the leader has no other go to contact the employees for final decision. Its not something the employees can take the final decision, it should be authorized by the leader, then it may get in to final decision. This style shows the good understanding between the employees and the leader. The employee recruited are highly skilled. Using this style will benefit the organization as well as it creates the good relation with the leader and employees. 2.1.The Selected theories of management and leadership have on organizational strategy: Under the autocratic leadership style in the organisation, The leader has the power of centralized decision making in the organisation. This is common to the directive style from organizational situational leadership Autocratic managers do not entertain any idea or initiatives from coworkers. The autocratic management is helpful to the managers because it gives a recognition to the team and organisation as well as it motivates the manager. It helps in quick decision making, as he is the only to decide for the whole team and the manager keeps decision to himself until he feels that is necessary to be shared to the coworkers. The autocrat tells others what not to do and what to do but not necessarily why it might be an appropriate model for the organisation where the team has low experience and skills or It has to be used in the effective way in the organisation with modern work forces. Participative or democratic style The democratic leadership style advantage decision making by the group as shown, such as first leader will set up the meeting with the group and he asks for the suggestions then he will give instructions to the group members. This style is more helpful for the managers so that the group member will be cooperative to the managers, and they may be motivated effectively and positively by the managers. The decision of the democratic leader is not unilateral as with the autocrat, because generally they used to discuss with group members and they will get a final idea with the group, and finally the manager will go for suggestion and ideas so that all the group members will participate by them. Democratic is not something is put to the vote it is the manager involves in to the participation of the group of members and make decision consulting with the group member and he has every right to take the own decision and cascade the final decision to the team members. If some one could not follo w or discuss with the team members then it may be a weak leadership. Free rein style or Laissez-faire A free-rein leader does not lead, leaves the group freely entirely on its own as shown; such as leader allows maximum liberal freedom to the group members, i.e they have given a liberal freedom and suggestions in choosing their own policies and procedure methods. 2.2.Leadership Stretegy Supports organizational direction: Its to remember the alternative situations for alternative leadership styles in the organisation. In the emergency time we have only little time only and where as in designated authority has more experience in the organisation or the rest of the whole team if they follow the autocratic leadership that would be more effective. In the case of highly motivated and designated team with a knowledge level of expertise, a more democratic or laissez faire style may be much more effective. When I complete the strategic leadership style survey I should remember that the leadership style has adopted and that would be the most effective and achieves the objective of the whole while balancing the interest of members of individuals. Also this leadership style survey is provided the answer but its not something we got the right answers, but I have the opportunities to ask the right knowledge questions in a right time about the leadership style at present and in the future cases. 3.1. Appropriate Method to review current leadership requirements: Jun Liu Business School, Renmin University of China, Beijing 100872, China Xiaoyu Liu School of Labor Relations Human Resources, Renmin University of China, Beijing 100872, China Abstract Leadership research has gone through several phases of development in the past 80 years or so. The paper identifies the major theories in each phase, and investigates the strengths and weaknesses of the research. Among those theories, transformational/charismatic leadership and leader-member exchange (LMX) are heavily discussed. The paper also discusses the future trend of research in leadership areas. Keywords: Review, Transformational Leadership, LMX Leadership can be defined as a process that an individual influences a group of individuals to implement strategies and achieve the collective goals (Yukl, 2002). Research in the area seeks to find out what types of leaders are likely to be successful and what factors determine leadership effectiveness. Generally, leadership research has gone though four paradigms: trait approach, behavioral approach, situational approach, and the contemporary theories of leadership. The paradigms of leadership theories shift with the progress from static to dynamic view of leadership with the trait and behavioral theories reflecting a personal, the situational theories an interpersonal, and the contemporary theories a relational approach to conceptualizing leadership. That is to say leadership has been examined as intrapersonal competencies, interpersonal processes, and relational dynamics. More specifically, I review those approaches, their strengths and weaknesses as followed: The trait approach The trait theories assumed that leaders were born, not made. In the literature, Stogdill (1948, 1974) completed two comprehensive reviews by synthesizing more than 200 studies of the trait approach. His two surveys identified a group 3.2.plan for the development of future situations requiring leadership -poor management performance -low   employee morale -unstable   organization structure -unsustainable business practices -high   employee turnover -unable to   manage diversity -Poor   Measured organizational performance improvement -Decreased quality of management -Poor   Healthy organizational climate- -Lower   morale -Increased   labor   turnover -Lack Organizational and personal growth -Poor teamwork -Lack   of   Retention strategies-talent leaving   the organization. -chronic industrial relations problems   -no means of resolving employee grievances   -increasing   employee turnover   -inter-group conflicts   -dissatisfaction with pay and conditions   -unclear job roles   -no clear performance measures   -poor recruitment standards / practices   -no management development programs   -no induction training for new employees   -critical skill shortages   -inter-departmental conflict   -lack   of   sufficient skills / power bases in the organization. -lack   of   appropriate leadership skills within the organization?   -lack   organization structure flexibilty   in the face of changing demands. staff have no clear roles and responsibilities?   -lack   of   procedures and management practices to facilitate the accomplishment of tasks. -lack   of   Ã‚  staff   potential spotting   Ã‚  and development   Ã‚  for the future. -lack   of   staff encouragement   to perform well through the giving of recognition, feedback, etc. -staff   do no   know what their expected performance standards are.   -organizations systems   do not encourage effective performance among your staff. -systems are   not   consistent across the organization. -there are   no clear rewards for effective performance within the   work group.   4.1. Developing leadership skills for a specific requirements Leadership has been described as the process of social influence in which one person can enlist the aid and support of others in the accomplishment of a common task.   Alan Keith of Genentech states that, Leadership is ultimately about creating a way for people to contribute to making something extraordinary happen. According to Ken SKC Ogbonnia, effective leadership is the ability to successfully integrate and maximize available resources within the internal and external environment for the attainment of organizational or societal goals. The above characteristics of being leaders; they are controlling and directing day to day business, they support the staff, they work as chefs and waiters and they try to achieve the best profit they can. 4.2. Useful methods to plan the developing of leadership skills Basic Skills in: Solving the problem and Making the decision Strategic Planning Delegation of work Internal Communications with the team Meeting Management with the mentor Managing Yourself with in the organisation

The Language Barrier for Puerto Ricans :: Caribbean History Language Essays

The Language Barrier for Puerto Ricans "Pollito, Chicken Gallina, Hen Lapiz, Pencil y Pluma, Pen. Ventana, Window Puerta, Door Maestra, Teacher y Piso, Floor I sing in English, I sing in Spanish, so all my friends can understand." The issue of language is central to the Puerto Rican experience in the United States. Living in a land where the dominant language is English, this Spanish speaking population is involved in a historical struggle to overcome the language barrier. Among other things, their unfamiliarity with the English language has been a major obstacle to the progression of the Puerto Rican people as a whole. The inability of Puerto Rican’s to speak English has served to exacerbate their situation in the United States; a situation where they are already met with discrimination simply for being foreigners. In the classrooms, Puerto Ricans have met only minimal success, largely due to their inability to properly communicate with teachers and peers. In the workplace, Puerto Ricans have historically been given only menial jobs. Due to their inability to speak English, many Puerto Ricans are unable to conduct themselves in job interviews, fill out application forms, or communicate with customers. As a result, the more competitive job fields show an under-representation of Puerto Ricans. Finally, many Puerto Ricans find it difficult to conduct themselves in places such as hospitals, courtrooms, and post offices due to the language barrier. This leads to the issue of bilingualism. Should the mainstream environment of the schools and workplace of America consist of two languages? T his issue has been debated for many years. This paper focuses on the issue of bilingualism in Hartford, while also looking at the context under which Puerto Ricans in Hartford find themselves in their current situation. These issues are examined with the use of historical fact, along with information and sentiments on current events in the Hartford community concerning the issue of bilingualism and culture. Puerto Rican History In the year 1508, the Spanish arrived in Puerto Rico and began the Spanish colonization of the island. At this time, the island was called Boriquen and was inhabited by an Indian tribe called Tainos. During this process, the Spanish established their way of life on the island while decimating the Tainos in terms of population due to Spanish disease, slavery and oppression.

The Ghost of Devils Den :: Ghost Stories Urban Legends Civil War

The Ghost of Devil's Den This ghost story was told by a nineteen-year-old Caucasian student at the University of Maryland. She is from the Baltimore Metro Area and lives with her mother and younger sister. I decided to approach her since she is a notorious lover of ghost stories and folklore. While we were hanging out with friends, I asked her to tell me a ghost story. As soon as I asked, her eyes lit up and she took me to the side, out of earshot of our friends. With great energy and enthusiastic facial expressions, she proceeded to tell me the following story about the Civil War site of the Battle of Gettysburg: My friend Carl was attending a reenactment of the Battle of Gettysburg. He went to a rocky corner of the battlefield filled with boulders, called Devil’s Den, to take a picture of the battlefield. He took out his camera and as he was about to take the picture, he heard a voice say, â€Å"What you’re looking for is over there.† Carl looked up and saw a man who resembled a hippie, wearing a floppy hat, no shoes and had long hair, pointing somewhere in the distance. Carl looked to see where the man was pointing, and when he looked back the man was nowhere in sight. The Storyteller claimed she once watched a documentary on television about the ghosts of Gettysburg, and it told a strikingly similar story of a ghost in a floppy hat saying the same words to many tourists who were taking pictures at Devil’s Den. Similar stories have been told involving a man in a floppy hat at Devil’s Den. One tells the story of a woman visiting the site of the Battle of Gettysburg. After experiencing no paranormal activity, she sarcastically challenged any ghosts of Devil’s Den to come home with her. A few days later she saw a man wearing a floppy hat and loose shirt in her house. She saw this vision many times, but it would always disappear very quickly. She believed this was a ghost from Devil’s Den accepting her challenge (U.S. Civil War History and Genealogy). Another version of the story involves a man who was also visiting the site of the Battle of Gettysburg. He took many photographs throughout the day. In the afternoon, a soldier dressed in a floppy hat, gray clothing and â€Å"possessing an odor of sulfurous gunpowder† approached him (U.

Morality and Ethics Essay

As two members of our group are children of sea captains, the case of Exxon Valdez oil spill was extremely interesting for our team. It demonstrates that irresponsible behavior of people can lead to the most devastating human-caused environmental disasters of the past century. Multiple factors have been identified as contributing to the incident. In our group’s opinion, the most important are: the captain was drunk being on duty, the third mate failed to properly maneuver the vessel, possibly due to fatigue or excessive workload and finally, the Raycas radar system was broken, because the company found it too expensive to fix it. To begin with, we think that due to the restricted responsibilities of the crew comparing to the captain, its commitment was relatively low. Therefore the main motivation was their salary and working conditions didn’t play a role. Probably that is why sailors were ready to work overloaded shifts in order to earn as much as they could, to our mind, despite the fact that physically it was impossible. Thus the responsible sailor had to assess his ability to steer the ship before beginning the watch – in this case he had no right to do that being too tired. Talking about the captain of the ship, our group found him being the most irresponsible person in this situation. He did not act according to the ethical norms. Firstly, captain is the person, who is fully responsible for the ship and the crew, but he dared to exceed the allowed norm of the alcohol. Secondly, he had to insist on the radar repair. Finally, he is the one who is responsible for the crew’s adequate performance. Nevertheless, Exxon is fully liable for this disaster and the subsequent oil spill being the owner of the tanker. Hence, in our opinion, the company had to pay for the cleaning up the oil spill. To sum up, our group sees the ethical dilemma only concerning the crew. They had two choices only. On the one hand, when signing the contract they automatically agree to fulfill their duties, that is why no one can afford to skip the watch even if he fills tired. On the other hand, the third mate had no right to expose to danger the ship and the rest of the crew by taking the watch in unacceptable condition. We would like to add, that despite the fact that this was the most devastating human-caused environmental disasters, it pushed the government to develop new laws in this industry and turned to be a good lesson for the human beings overall.

Zaara Fashion House – New Year Promotional Campaign

Fashions is an exclusive fashion retail store of Bangladesh. It retails women, men, and kids fashionable apparels. Located in a prime zone of Goulash-l in Dacha, it gives customers freedom of choice regardless of their age, location, taste, season, and fashion persona. Established and inaugurated in August 2009, the retail store KARA Fashions has successfully placed itself among the most celebrated and recognized retail stores of the country. The store produces an exclusive array of products and outfits.Most of these are designed and manufactured in house, while he rest are exclusively commissioned from the most talented craftsmen and weavers around the world. Its unique design and features symbolize aristocracy, and target the most elite customers of the country. Thus, KARA Fashions compliments a trendy lifestyle, and always ranks itself one step ahead with the latest styles and designs. Product Offerings KARA Fashions offers different ethnic and aristocratic products to their high end customers. They usually focus on the classy, gorgeous and ethnic products which will quench the aristocratic thirst of their potential customers.The main products that KARA Fashion offer are- Scares, Salsas Kamikaze, Sherwin, Suit, Shirt, Men's accessories, Kids Wear, Jewelry, Cosmetics, Ladies Footwear, Ladies Bag etc. 2. Mission The mission of Kara Fashions Ltd. Is to provide their customers with exclusive products in order to compliment a trendy lifestyle. Thus they refer it as ‘A House of Exclusive Fashions. ‘ 3. Vision Kara Fashions Ltd aims to practice relationship marketing which involves creating, maintaining, and enhancing long-term relationship with their each and every customer. Thus, they believe to stay always one step ahead with the latest styles and designs. . Situation Analysis Today's market is characterized by highly competitive organizations which are all vying for consumer's loyalty. Firms are faced with the challenge to maintain their own competi tive edge to be able to survive and be successful. Strategies are carefully planned and executed to gain the ultimate goal of all: company growth. However, external factors are not the only elements which influence growth. There are also internal factors, components working within the organization which shape the direction of the company. The company's marketing environment influences the business organization directly.This includes the suppliers that deal directly or indirectly to the people within the business organization, the consumers and customers who demand for the products or services offered by the company, and other local stakeholders who influence the decision-making process or affected by the business decisions made by the business firm. As such, the marketing environment of a particular company describes the relationship between firms and the driving forces that control this relationship who are members of a specific industry wherein the relationship is local and the fi rm may exercise a degree of influence.The situation analysis of KARA Fashion House is described according to two types of environment in exists in- 1. Micro Environment 2. SOOT 3. Macro Environment Situational Analysis Figure 1. 1 The Micro Environment The term micro-environment denotes those elements over which the marketing firm has control or which it can use in order to gain information that will better help it in its marketing operations. In other words, these are elements that can be manipulated, or used to glean information, in order to provide fuller satisfaction to the company's customers.This is accomplished through the manipulation of the variables over which a company has control in such a way as to optimize this objective. 1. 1. 1 The Four As' and the marketing mix The Four As' stands for: 1. Product 2. Price 3. Place 4. Promotion Product- is the market offering. The products KARA offers are exclusive scares, salsas zamia, branded cosmetics, Jewelry, suits, hand bags, s hoes, kids wear and much more. All the products offered are of very high quality. Every product is carefully checked before putting into display.Price- in this context price refers to each KARA product costs. Prices of these reduces are set on the basis of product quality, brands as well as market competitiveness. Place- the location of KARA Fashion House is a great advantage of it. Goulash is one of the most prime and posh area of Bangladesh. So KARA stands in very close distance of the target market. Promotion- the promotion and campaigns would following parts. 1. 1. Employee be describe De elaborately in the KARA Fashion has a very strong line of efficient employees.They are highly dedicated towards works. For better Job involvement and Job satisfaction KARA pays them with a scale higher than the regular salary scale. Moreover, compensations, bonuses, health insurance, performance appraisals are also given in expectation of higher motivation. KARA has around 50 employees working in it. In maintains a great mix of both full time and part time workers whereas 20 are full timers and the rest are part timers. But regardless of everything else KARA ensures they are equally well behaved and helpful towards all the customers.So in a sense, these highly efficient bunches of employees are the greatest resources of KARA Fashion House. 1. 1. 3 Supplier This consists of other business firms or individuals who provide the marketing firm tit raw materials, product constituents, services or, in the case of retailing firms, possibly the finished goods themselves. The buyer/supplier relationship is one of mutual economic interdependence, both parties relying on the other for their commercial well-being.Although both parties are seeking stability and security from their relationship, factors in the supplier environment are subject to change.

Region IX – Zamboanga Peninsula

Recently we were told by our teacher to pay a visit at our campus on the occasion of a science fair giving each one of us one ticket. Accordingly, I went there and enjoyed a lot. What I saw was a fantastic arrangement of exhibits done by the students. I saw miniatures mostly about agriculture. One of the unique creations was a miniature of Terrace Farming, it is a piece of sloped plane that has been cut into a series of successively receding flat surfaces or platforms, which resemble steps, for the purposes of more effective farming.As we all know, one of the popular terraces is he Banana Rice Terraces (Haggard-Haggard Palatal). Some of the miniature was describing about Contour Plowing it is plowing across a slope following its elevation contour lines. This is the kind of agricultural solution used by most farmers as a tool for weed control, prevent soil erosion, and in some cases to promote plant growth. There was also a miniature about Interloping meaning growing two different veg etables in an area at the same time to save space. On the other miniature it was about how to farm organically.According to a book that I have read, eating organic odds may in fact, reduce the risk of heart attacks, strokes and cancer for individuals who abstain from consuming products produced by conventional farming methods. I also saw a miniature about animal farming. Mostly the main products of this Industry are meat, milk and eggs for human consumption. Here's to other miniature about urban Vile which pictures out the setting of an urban place or what we call a city where you can see tall buildings and establishments and a large crowd of people.In the contrary there was a miniature with details of a Rural place where It Is located outside the city with a low population density and small settlements. And lastly, one object drew my attention. It was about family farming. Like In my hometown we have a small farm at the back of our house, and planting Is one of our ways of bonding during weekends. One of the Important features of family farms Is the Intimate connection between families and farms which creates vital links between a farm and the food being grown. I miss home. I spent full 30 minutes rooming around the science fair enjoying such wonderful creations.

My Family and the Conservative Theory Essay

I believe that my family is a great example of the conservative theory. I was raised by both of my parents who have been married for over thirty years. There was myself and my two sisters that also comprised our household. We lived in Saudi Arabia growing up and are followers of Islam. My father, Mahdi was a geography teacher on the college level, from which he has since retired. My mother taught middle school science. My older sister has been married for over five years and has one three year old child. I am currently attending college and studying finance and my younger sister is attending college and studying accounting. Being part of the Muslim community is part of the conservative nature of my family. We choose to try and live our lives according to the five pillars of Islam. These are the beliefs that the religion was founded upon. The first pillar of Islam is that Allah is complete and supreme oneness and that Muhammad was the great prophet. This one pillar is the foundation for the way that my family chooses to live our lives. We choose to participate in a culture where we are governed by Allah a will to do what is good and morally just. The second pillar of Islam is the establishment of daily prayers. Daily prayers are very important in our culture. They are the communication that we have with Allah and the way that we are guided in how we are going to make decisions. As a follower of Islam we pray five times per day. The third pillar of Islam is concern for the needing. This includes being willing to sacrifice to help others who are less fortunate than we are. It is important in my culture to give to those who are needy and to help them as much as we possibly can. Islam teaches not being selfish and wanting worldly possessions but rather being selfless and always giving of oneself to others. This means that it has never been important to me or my family as to whether or not we are wearing a certain brand of clothing or whether or not we have certain processions. It is not important for us to try to keep up with mainstream society in an attempt to fit in as we believe that our riches are destined for another time and another place. We value saving and are conservative in our spending and shopping habits. The fourth pillar of Islam is self purification through fasting. Fasting is a big part of our beliefs and we are conservative in this area as well. We fast during the month of Ramadan from sun up until sun down. This is to teach us discipline. There is no stronger conservative value than discipline as when we are disciplined we are able to make decisions without feeling the pressure of the outside world. The fifth pillar of Islam is to make a pilgrimage to Mekkah. The pilgrimage to Mekkah is important as we are traveling to the divine spot where Muhammad heard the words of Allah that he used to write the Koran. One of the biggest differences in the Islamic culture that we are a part of in comparison to most other cultures of today is the beliefs that we are taught about dating. Muslim teachings teach us that we are not to date until we are in the confines of marriage. It is against our teachings to go out on dates or to have premarital sex as these behaviors are considered worldly and a poisoning of ourselves and we are to be a temple to Allah. In our culture we start a dua when we want to start dating. This means that we ask Allah to send us a mate with whom we will make a good match with. We believe that Allah’s divine wisdom will allow us to meet our perfect match. I believe that a man and woman are created to be able to find the one perfect match for you who will give you spiritual unity and peace. When someone in my family is looking for a partner with whom to share their life with, the whole family becomes involved. It is still important to find someone who we are going to connect with and get along with and having a person chosen for us is not a way of controlling who we date but rather a way of keeping us from making the mistake of bad relationships and having to suffer through the pain of heartache. The overall idea is that by keeping ourselves pure and clean and not being in a physical relationship with anyone we are able to find a partner who truly is the best match for us emotionally. We also are able to find the one true partner that Allah has created for us by not letting physical attraction or other decisions get in our way. We remain pure for those who we are going to marry and yes this does mean that we do not believe in premarital sexual intercourse or sexual relationships in any way. My family is very conservative in the area of dating as both my sister and her husband and my mother and father are part of marriages that were arranged. My family also does not believe in divorce. We believe that if you truly are with the person that Allah has placed you with then there is no reason for divorce from that person. Growing up following the practices of Islam has made me a much more conservative person than most of my peers. I believe that those who commit crimes should be punished to the full extent. One heated subject for today’s culture has been the idea of corporal punishment and I am in favor of this practice when it is used for the correct reasons and within the realms of the law. I feel that this is the conservative approach on this topic, following the adage if you â€Å"can’t do the time then don’t do the crime. † I also am against abortion which is another topic of heated debate today. Abortion is something that is not allowed through the practices of Islam and something that would never be okay for me or anyone in my family. Homosexuality is also popular today and in the faith of Islam it is not allowed as it is considered a true sin. However it is also a sin to perform any type of sexual act outside of marriage as sexual acts are supposed to be for the purpose of creating a family. My family has not had children out of wedlock. My family has always worked hard to uphold themselves to the moral standards of Islam. Some of this has been difficult as I have always had to think of the moral debate to a subject and how I could justify my decision within my faith. I was raised to be respectful to my parents and could not have said some of those things that I have heard other students talk about saying to their parents. I have always been raised that without my parents I would not be anything and that I needed to uphold the utmost respect for them and their decisions in life. Even when I have disagreed with things that my parents have said, I will still give them the respect of listening to their decision without question. I have always known that my family was different from the other families that I have been witness to since moving to the US. Since we have always practiced Islam and since I grew up in Saudi Arabia I did not realize that we were so much more conservative than other families. Being a part of a conservative family has taught me many things, including that I have values and principals that I am not willing to let down for any reason. I want to follow the teachings of Allah and I want to preserve a culture that I feel reacts in a better way too many of the controversial issues of today. There are all kinds of issues that have to be dealt with on a regular basis when someone is growing up in today’s culture. In a way it is nice to not have to worry about some of these issues as they are behaviors that are not permitted by Islam therefore they are behaviors that I have never thought about being involved in. I do not date and I am a virgin and therefore I find that I am a part of a subgroup within the US culture that is unique in itself. Stating that I am simply conservative might be an understatement as I believe that Allah has better for me and that I should uphold myself to the highest moral standards in order to be able to please him and find my end reward later after this life has ended. I also believe that I have been taught unique values which have carried over into my conservative way of thinking. I was raised by both of my parents who are still married and I have watched my older sister attempt to have the same life that we did growing up. I am hopeful that one day Allah will speak and that I will find my mate and be able to fulfill my life as well.